Each club is assigned an Assistant District Governor, or “A.G.”, who is responsible for about four clubs each and acts as a liaison between the Club and the District. Clubs communicate priorities up to the District level and ask for help locating District-level resources, and District officers communicate their priorities and requests to Clubs, largely through the Assistant Governors.
 | Assistant Governor Area 2 Susan Tuggle Club: High Ridge Area 2 Clubs: Arnold, De Soto, Festus-Crystal City, High Ridge Contact |
 | Assistant Governor Area 3 Dennis Hartmann Club: Union Area 3 Clubs: Rotaract - East Central College, Rotary Club of Washington, Sullivan, Union Contact |
 | Assistant Governor Area 5 Rennie Davis Club: Vandalia Area 5 Clubs: Mexico, Paris, Vandalia Contact |
 | Assistant Governor Area 6 Tilghman J. Feeler Club: Sikeston Area 6 Clubs: Bell City, Dexter, Sikeston Contact |
 | Assistant Governor Area 7 George H. Wrape Jr. Club: Cape Girardeau West Area 7 Clubs: Cape Girardeau, Cape Girardeau County, Cape Girardeau West, Jackson Contact |
 | Assistant Governor Area 8 Kimberly (Kim) Ann Combs Club: Piedmont Area 8 Clubs: Ellington, Piedmont, Poplar Bluff Contact |
 | Assistant Governor Area 9 Susan J. Cobb Club: Cottleville/Weldon Spring Area 9 Clubs: Florissant, Rotaract - Saint Louis Univ., St. Louis, Webster Groves, West St. Louis County Contact |
 | Assistant Governor Area 10 Tim Jenkins Club: Louisiana Area 10 Clubs: Bowling Green, Hannibal, Louisiana Contact |
 | Assistant Governor Area 11 Pamela J Harrell Club: Troy Area 11 Clubs: O'Fallon Missouri, St. Peters, Troy Contact |
 | Assistant Governor Area 12 Donna Marie Pierre Club: Wentzville Area 12 Clubs: Montgomery County, Warren County, Wentzville Contact |
 | Assistant Governor Area 13 Nelda K Morgan Club: Kennett Area 13 Clubs: Hayti, Kennett, Steele Contact |
 | Assistant Governor Area 14 Basil Zarkadis Club: St. Charles Area 14 Clubs: Cottleville/Weldon Spring, St. Charles, St. Charles-Sunrise Contact |
 | Assistant Governor Area 15 Valerie Sorensen Club: Cottleville/Weldon Spring Area 15 Clubs: Chesterfield, Clayton-Ladue, Overland Contact |