Each club is assigned an Assistant District Governor, or “A.G.”, who is responsible for about four clubs each and acts as a liaison between the Club and the District. Clubs communicate priorities up to the District level and ask for help locating District-level resources, and District officers communicate their priorities and requests to Clubs, largely through the Assistant Governors.
| Assistant Governor Area 1 Stephanie Gremminger Club: Ste. Genevieve Area 1 Clubs: Perryville, St. Francois County/ Bonne Terre, Ste. Genevieve Contact | |
 | Assistant Governor Area 2 Susan Tuggle Club: High Ridge Area 2 Clubs: Arnold, De Soto, High Ridge Contact |
 | Assistant Governor Area 3 Dennis Hartmann Club: Union Area 3 Clubs: Rotaract - East Central College, Rotary Club of Washington, Sullivan, Union Contact |
 | Assistant Governor Area 4 John Higdon Club: Crestwood-Sunset Hills Area 4 Clubs: Crestwood-Sunset Hills, Forest Park , Kirkwood Contact |
| Assistant Governor Area 5 Rennie Davis Club: Vandalia Area 5 Clubs: Mexico, Paris, Vandalia Contact | |
 | Assistant Governor Area 6 Tilghman J. Feeler Club: Sikeston Area 6 Clubs: Bell City, Dexter, Sikeston Contact |
 | Assistant Governor Area 7 Thomas Joseph Pujol Club: Cape Girardeau West Area 7 Clubs: Cape Girardeau, Cape Girardeau County, Cape Girardeau West, Jackson Contact |
 | Assistant Governor Area 10 William (Bill) James Dees Club: Hannibal Area 10 Clubs: Bowling Green, Hannibal, Louisiana Contact |
 | Assistant Governor Area 11 Raymond Randolph Davis Club: Troy Area 11 Clubs: O'Fallon Missouri, St. Peters, Troy Contact |
 | Assistant Governor Area 12 Donna Marie Pierre Club: Wentzville Area 12 Clubs: Montgomery County, Warren County, Wentzville Contact |
 | Assistant Governor Area 15 Harry E. Tart Club: St. Charles Area 15 Clubs: Cottleville/Weldon Spring, St. Charles, St. Charles-Sunrise Contact |
 | Assistant Governor Area 16 Dan Faulkner Club: Cottleville/Weldon Spring Area 16 Clubs: Chesterfield, Clayton-Ladue, Overland Contact |